The first lesbian vampire movie?
Yes, I know it's ultimately revealed that Luna isn't a real vampire, but for the majority of the film's running time we're lead to believe she is... and despite all the various male characters running around, it's Irena who Luna's exclusively interested in. The scenes featuring the two of them are undeniably sexually charged.
I adore the sequence when Luna (behaving more like a lover than a predator) approaches Irena and tenderly touches her, whereupon an overwhelmed/frightened Irena instinctively backs away & sits down, and Luna silently tries to comfort & reassure her. Fedor then interupts, causing Luna to hiss cat-like at him, grab Irena by the hand and protectively bundle her away. The entire scene is spellbinding.
(Incidentally, Luna's hiss was apparently an ad-lib. All female vampires have hissed cat-like ever since, thanks to Caroll Borland.)
Since Mark of the Vampire was released the year before Dracula's Daughter (1936) - in which the title character preys upon a nervous street-walker called Lili (played by Nan Grey) in a scene heavy with sexual tension - this means that Mark can almost certainly claim to be the first lesbian vampire movie.
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