The saddest moments... [spoilers]
There is quite a few really sad moments throughout the series...
The other day when I was watching it I was thinking about Yoshii.
So he came back from the surface. Was he the LAST trully living person on the surface?
Since everyone else was a sort of ghost/hollogram, and there did not seem to be any real people, that means that Yoshii must have been a bit lonely and bored up there, all by himself, which is kind of terryfying.
Also, when the doctor commited suicide, with her voice on the radio.