The unabridged list of “DwD” villains

Okay, here we go (in order of first appearance):

*Taurus Bulba - A bull and the first villain Darkwing ever fought. He didn't have any super powers (besides extreme intelligence, strength, and ruthlessness) until F.O.W.L. rebuilt him as a cyborg following an explosion that nearly killed him. Has four assistants - a goat named Hammerhead, a donkey named Hoof, a ram named Mouth, and a cow named Clovis. Appears in the episodes "Darkly Dawns the Duck," and "The Steerminator."

*Bushroot - A botanist duck who, after his funding was cut, accidentally mutated himself into a half-plant through an experiment gone wrong. He now is able to communicate with plants telepathically, and grow back from most damage. Although he can be dangerous at times because of said powers, he's definitely Darkwing's least evil recurring enemy - he's very lonely and often expresses his need for a friend. Best friend/henchman/pet is a large Venus Flytrap named Spike, who acts like a dog. Appears in the episodes "Beauty and the Beet," "Night of the Living Spud," "Easy Come, Easy Grows," "Just Us Justice Ducks," "Life, the Negaverse, and Everything," "Darkwing Doubloon," "It's a Wonderful Leaf," "Twin Beaks," "The Incredible Bulk," "A Star is Scorned," "Jail Bird," and "Slime Okay, You're Okay."

Ammonia Pine - I believe this is the "fat chicken lady" you were referring to. She is an agent of F.O.W.L. (the Fiendish Organization for World Larceny) after being exposed to an experimental cleaning chemical that twisted her brain. She has some obsessive-compulsive traits, and her main weapons are her mops. Appears in the episodes "Dirty Money," "Cleanliness is next to Badliness," and "Dirtysomething."

Megavolt - The villain who appeared in the most episodes. Of unknown species (presumably some kind of rodent), he was a harmless science nerd until one of his high school experiments was sabotaged by bullies, frying his brain, giving him electrical powers, and (although still a genius)leaving him insane. How insane? Well, he can't remember his own name, talks to electronics, and is convinced light bulbs are enslaved by mankind and must be "rescued." He seems to have two basic moods: very angry and very confused. Yet there are times when he will team up with Darkwing, and he seems very proud of himself whenever he manages to save the day. Appears in the episodes "Duck Blind," "Comic Book Capers," "A Revolution in Home Appliances," "Just Us Justice Ducks," "Up, Up, and Awry," "Life, the Negaverse, and Everything," "Darkwing Doubloon," "Twitching Channels," "Dead Duck," "Time and Punishment," "Stressed to Kill," "Inside Binkie's Brain," "Jail Bird," "Negaduck," "Whirled History," "Inherit the Wimp," "The Frequency Fiends," and "Clash Reunion."

Steelbeak - F.O.W.L.'s top agent, a rooster with a metal beak (it's unknown why he has it, although it is known that F.O.W.L. gave it to him). He is intelligent and fairly ruthless, so he's one of Darkwing's more dangerous enemies. He's pampered by F.O.W.L. and almost always seems calm and in control, but at times he will snap and become incredibly violent. Always seems to be accompanied by Eggmen, none-too-bright thugs dressed in helmets and jumpsuits. Appears in the episodes "Water Way to Go," "Trading Faces," "Bearskin Thug," "Cleanliness is next to Badliness," "Smarter than a Speeding Bullet," "The Darkwing Squad," "Bad Tidings," "Tiff of the Titans," and "The Steerminator."

Tuskernini - A walrus film director who defines the word "ham," he commits crimes in the style of movies or while making movies. Always travels with a group of silent-but-efficient penguin henchmen. Appears in the episodes "Film Flam," "Slaves to Fashion," "Hush, Hush, Sweet Charlatan," "Adopt-a-Con," and "A Duck By Any Other Name."

Jambayla Jake - A highly annoying (and sterotypical) boy-from-the-bayou, he occasionally drops by the "Big City" to hassle Darkwing, accompanied by his pet alligator, Gumbo. Appears in the episodes "Can't Bayou Love" and "Double Darkwings."

Quackerjack - A duck who dresses in a jester outfit. He used to be a (presumably harmless) toymaker until video games put him out of business and drove him crazy. He carries around a little banana puppet named "Mr. Banana Brain," and converses with it regularly, supplying the doll with a high-pitched voice. He seems to be unaware of the doll lacking life of its own. He attacks with dangerous toys, such as toy soldiers that fire real bullets, giant crying dolls that can flood entire cities, or the ever-popular large pairs of chattering teeth with homing devices in them. He can be more pyschotic and dangerous than his silly, buck-toothed appearance implies. However, unlike the Joker, he has moments of geniune, childlike innocence, which makes him one of my personal favorites. Appears in the episodes "Whiffle While You Work," "Just Us Justice Ducks," "Days of Blunder," "Life, the Negaverse, and Everything," "Toys Czar Us," "Darkwing Doubloon," "Quack of Ages," "Time and Punishment," "Stressed to Kill," "Jail Bird," and "The Haunting of Mr. Banana Brain."

Negaduck* - Darkwing's evil double from the Negaverse. He looks exactly like Darkwing but dresses in red, black, and yellow, and is pretty much as close as the Disney Afternoon could get to evil incarnate. He seems to have an endless supply of weapon; his favorite appears to be his chainsaw. He runs the Fearsome Five, a (not always very effective) villains team consisting of himself, Megavolt, Liquidator, Quackerjack, and Bushroot. In the Negaverse, he lives with a violent Nega-Launchpad and a sugery-sweet Nega-Gosalyn. *Not to be confused with The First Negaduck (explained below.) Appears in the episodes "Just Us Justice Ducks," "Life, the Negaverse, and Everything," "The Secret Origins of Darkwing Duck," "Disguise the Limit," "Darkwing Doubloon," "My Valentine Ghoul," "Let's Get Respectable," "The Quiverwing Quack," "Jail Bird," "Bad Luck Duck," "Going Nowhere Fast," and "Malice's Restaurant."

*The First Negaduck - When Megavolt hit Darkwing with his Tron-Splitter, he seperated him into two ducks, his good side (Posiduck) and his bad side (Negaduck). Darkwing was put back together in the end with no side effects. This Negaduck has not been seen again, and he is not the same as the Negaduck listed above. Appears in the episode "Negaduck."

The Liquidator - A dog made entirely of water. He was once a bottled water salesman named Bud Flud, who was sabotaging competator's water during a heat wave. When Darkwing caught him, he accidentally pushed him into a vat of contaminated water, turning him into The Liquidator. Liquidator has power over all water and is able to take on any shape or size he wants. He's mainly into crime for the money, and continues to talk like a salesman. Appears in the episodes "Dry Hard," "Just Us Justice Ducks," "Life, the Negaverse, and Everything," "Darkwing Doubloon," and "Jail Bird."

Molitary - A mole who wants to wipe all surface-dwellers off the face of the Earth. Appears in the episodes "Aduckyphobia" and "That Sinking Feeling," plus a minor role in "Kung Fooled."

Morgana - Yes, Morgana McCawber, Darkwing's girlfriend. She was once a criminal, getting into crime for the money to pay off her student loans. She was reformed, however, by her great love for Darkwing. Her constant companions are her bats, Eek and Squeak, and her spider Archie. She is a sorceress with magical powers (unforunately for Darkwing when he happens to put his foot in his mouth). Appears in the episodes "Fungus Amungus" and "Ghoul of my Dreams" as a villain, and "Just Us Justice Ducks," "My Valentine Ghoul," "Dead Duck," "Monsters R Us," "Hot Spells," and "Malice's Restaurant" as a good guy.

The Brainteasers - A group of alien hats who are able to use other living organisms as "hosts," and are bent on interplantary domination. Their leader is Flarg the Terrible, and his two assistants are Barraba and Nickto. He also has a girlfriend named Talaya. Appear in the episodes "Battle of the Brainteasers" and "Revenge of the Return of the Brainteasers, Too!"

Splatter Phoenix - A duck artist who wants to shock the world with her "darling innonative neo-postmodern" something-or-other. Unfortunately, she's broke, so she's turned to crime. She has a magic paintbrush which allows her to enter paintings or paint things that come to life. Appears in the episodes "A Brush with Oblivion" and "Paint Misbehavin'."

VILLAINS THAT APPEARED IN ONLY ONE EPISODE:

Lilliput - "Getting Antsy"
Major Trenchrot - "Apes of Wrath"
The King - "Paraducks"
Jock Newbody - "You Sweat Your Life"
Wacko - "When Aliens Collide"
Dr. Fossil - "Jurassic Jumble"
Isis Vanderchill - "All's Fahrenheit in Love and War"
Noddoff - "Ghoul of my Dreams"
Major Synapse - "Heavy Mental"
Ordinary Guy - "Planet of the Capes"
Phineas Sharp - "In Like Blunt"
Darkwarrior Duck - "Time and Punishment"
Paddywhack - "The Haunting of Mr. Banana Brain"
Ample Grime - "Dirtysomething"
Neptunia - "Something Fishy" (reformed) "Just Us Justice Ducks"
Camille Leon - "Calm a Chameleon"
Weaselman - "The Merchant of Menace"
The Devil - "Hot Spells"
Bianca Beakley - "Fraudcast News"
Cement Head - "Mutancy on the Bouncy"
Johnny T. Rex - "Extinct Possibilty"

Is that everyone? Yep, I think so. Okay, hope this helps!