perhaps Isabel had actually died ?
I doubt it, because we see her in another scene later. She's laying in bed, and sees a shadow on the wall that's a combination of the post in the middle of the window and some tree branches outside. A child would most likely be scared, but Isabel just pulls the blankets up over her head.
The implication seems to be that she's left childhood (or childhood's left her).
...we never see Ana talk to Isabel again ?
We never see nor hear about Ana talking to or recognizing
anybody again. It's not just an Isabel thing.
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It seems quite possible Isabel was primed for a "sexual awakening" and chose to begin sleeping in her own room, effectively "leaving childhood". She appears to be fairly near the age of puberty. Remember her scene earlier with spreading a drop of blood on her lips and looking at the effect in a mirror? Or the scene with both sisters washing up for school where she showed her fascination with shaving? The break in her relationship with Ana could have provided a convenient time to do something she had been leaning toward anyway.
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