At least two live snakes are used. You can see the first snake's tongue flickering in and out and its body moving proving that it is not a prop. However, when the knife is pressed down on the snake, we do not actually see the head being cut off. That happens off camera or the snake we saw was not actually killed. After that shot we see a shot of the still-moving body and still-moving severed head of a formerly live snake that was just decapitated, which may or may not have been the first snake. When the scientist tells the journalist to touch the snake and we see the head of the snake move in reaction to his hand, the head and body were not in the same position we saw previously. That's because they used the decapitated body of one snake, and put the head of a second live snake of the same species through a hole next to the body to make us think that it was the severed head that was still alive. After the head moves we can see the snake's tongue flickering in and out, proving that it is the head of a live snake and not the recently decapitated head.
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