Self Sacrifice?

Well, Princess Yuki's elders - when the enemy finds out their hidden fortress, they burn it down (so that the smoke could be a single) and die in the process (so that they will not have to reveal where she and her entourage are headed).

Mifune, himself throughout the whole journey. A less loyal samurai would probably flee with any gold he can carry and of course Mifune’s sister playing a double for the princess (seen off screen though) eventually gets executed.

The woman they picked up offers herself as a shield to the princess when they are caught in gunfire.

And in the end, our two leads Tahei and Matakishi, who have been greedy throughout the picture, yet now each is now willing to give that bar of gold to the other (remember, they've lost everything).